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). Inside the free of charge ChVLig-AMP, the OB area is reflected far from the NTase area to completely expose the DNA-binding area higher than the AMP-binding pocket. The peptide section that is destined to be the latch is disordered inside the no cost ligase and delicate to proteolysis, but this segment is shielded from proteolysis when ChVLig binds to nicked DNA.
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